建立及优化肠炎沙门菌的诱导耐酸反应模型

doi:10.11843/j.issn.0366-6964.2018.02.020

摘要/Abstract

摘要：

本研究旨在研究肠炎沙门菌的酸耐受性。应用Box-Behnken设计-响应面法创造最优条件评估沙门菌在乙酸创造的酸性压力的条件下的活动，对比优化前后肠炎沙门菌生存能力的变化，以及耐酸基因相对转录的变化，并将此模型应用于其他常用酸创造的酸性压力条件下，诱导肠炎沙门菌的耐酸性，对比耐酸基因相对转录的差异。结果显示，最佳工艺条件：培养温度22.5℃、培养时间6 h、菌液添加量15%。验证试验显示，细菌菌落数为3.966×10⁸ CFU·mL^-1，实测值与预测值接近。同时该模型环境下，肠炎沙门菌的生存能力最高，耐酸基因的相对转录升高最为显著（P<0.01）。应用于其他常用酸创造的酸性压力条件下，同样酸耐受基因的转录升高最显著（P<0.01）。研究结果说明响应面法建立的模型预测性良好，能合理地优化细菌酸耐受诱导条件。

Abstract:

This study was designed to investigate effects of acid on survival of Salmonella enteritidis. In this study, Box-Behnken design with response surface methodology was used to create the optimal conditions to evaluate the growth of Salmonella under acetic acid pressure environment. The changes of S. enteritidis survivability and the relative expression of acid tolerance-related genes were compared before and after optimization. This model was also used to compare the different relative expression of acid tolerance-related genes in S. enteritidis which induced by other common acids. The results showed that the optimum favorable conditions were as follows:incubation temperature 22.5℃, shaking time of 6 h, amount of bacteria added 15%.Validation experiments showed that bacterial colonies count were 3.966×10⁸ CFU·mL^-1, which basically met with the standard value. At the same time, the survival of S. enteritidis was highest in this environmental condition, and the relative expression of acid tolerance genes were significantly higher (P<0.01). The expressions of acid tolerance genes were also significantly higher (P<0.01) under acidic stress conditions created by other commonly used acids. Our study indicates that the model established by response surface methodology has a reliable tool which could be reasonably used in bacterial acid tolerance induction conditions during optimization.

中图分类号:

S852.61

汪燕秋, 吴丽云, 陈晓刚, 孙彤, 李冲, 俞道进. 建立及优化肠炎沙门菌的诱导耐酸反应模型[J]. 畜牧兽医学报, 2018, 49(2): 401-411.

WANG Yan-qiu, WU Li-yun, CHEN Xiao-gang, SUN Tong, LI Chong, YU Dao-jin. Establishment and Optimization of the Acid Tolerance Response Model for Salmonella Enteritidis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 401-411.

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